ligation

Ligation can also be performed in any of the four restriction endonuclease NEBuffers or in T4 Polynucleotide Kinase Buffer if they are supplemented with 1 mM ATP. uang pecahan 50000 baru Room Temperature Ligation For convenience, ligations may be done at room temperature (20-25°C). fifa world cup sampai tanggal berapa For cohesive (sticky) ends, use 1 µl of T4 DNA Ligase in a 20 µl reaction for 10